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yeast stress granules (Pab1-GFP) and P-bodies (Edc3-mCh) following glucose deprivation
Project Image
human stress granules (G3BP1-GFP) and P-bodies (Dcp1a-RFP) following arsenite stress

Stress granules and P-bodies are cytoplasmic biomolecular condensates conserved throughout eukaryotes. They consist of non-translating mRNAs, RNA-binding proteins, and many additional proteins, RNAs and small molecules. They are induced under conditions of cellular stress, where they may facilitate cell survival as part of the integrated stress response. Dysregulation of stress granule assembly and clearance in particular is linked to the pathology of various cancers and neurodegenerative diseases, including ALS. 

Though much is known about stress granule and P-body assembly mechanisms and composition, their underlying functions and how cells clear them remain poorly understood. These topics are of key interest to our lab.


J. R. Buchan
Stress granule and P-body clearance: Seeking coherence in acts of disappearance
Seminars in Cell & Developmental Biology 159-160 10-26 (2024)
J. J. Bearss, S. K. Padi, N. Singh, M. Cardo‐Vila, J. H. Song, G. Mouneimne, N. Fernandes, Y. Li, M. R. Harter, J. M. Gard, A. E. Cress, W. Peti, A. D. Nelson, J. R. Buchan, A. S. Kraft, and K. Okumura
EDC3 phosphorylation regulates growth and invasion through controlling P‐body formation and dynamics
EMBO reports 22 (2021)
N. Eshleman, G. Liu, K. McGrath, R. Parker, and J. R. Buchan
Defects in THO/TREX-2 function cause accumulation of novel cytoplasmic mRNP granules that can be cleared by autophagy
RNA 22 1200-1214 (2016)
J. R. Buchan
mRNP granules
RNA Biology 11 1019-1030 (2014)
J.  . Buchan, R. Kolaitis, J.  . Taylor, and R. Parker
Eukaryotic Stress Granules Are Cleared by Autophagy and Cdc48/VCP Function
Cell 153 1461-1474 (2013)
J. R. Buchan, J. Yoon, and R. Parker
Stress-specific composition, assembly and kinetics of stress granules in Saccharomyces cerevisiae
Journal of Cell Science 124 228-239 (2011)
J. R. Buchan, and R. Parker
Eukaryotic Stress Granules: The Ins and Outs of Translation
Molecular Cell 36 932-941 (2009)
J. R. Buchan, D. Muhlrad, and R. Parker
P bodies promote stress granule assembly in Saccharomyces cerevisiae
The Journal of Cell Biology 183 441-455 (2008)